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WebPCR was performed under the following conditions using a PCR machine (C1000 touch Thermal cycler; Bio-Rad, Hercules, CA, USA): denaturation at 95 °C for 20 s, annealing at 56 °C for 30 s, and extension at 72 °C for 30 s for 35 cycles and, final extension at 72 °C for 5 min. The human (h) and mouse (m) specific primers used are the followings: WebA tissue transglutaminase IgA (tTg-IgA) test is used to help doctors diagnose celiac disease. In this autoimmune disorder, the immune system mistakenly thinks that gluten — a protein in wheat, barley, rye, and oats — is a foreign invader. It makes antibodies that attack an enzyme in the intestines called tissue transglutaminase (tTG). WebFinal answer. Transcribed image text: What spquence of amino acids will be generaled from the given BWAd belgw + straind 5 ATC TCT TCG TTA TCC TTG 3' a strand 3'TAC AGA AEC AAT AGG AAC I. decimals corbettmaths worksheet